Also known as the Cenacle, the Upper Room is held by many Christians to be the site of the Last Supper, though its authenticity is highly disputed. More than just the site of the Last Supper, Christians believe that the Upper Room is also the site where Jesus washed his disciples’ feet, where Jesus appeared after his resurrection, and where the disciples met after the Ascension to elect of Matthias as Judas’ successor. Finally the Upper Room is associated with the appearance of the Holy Spirit at Pentecost.
For more information, please visit the Wikipedia entry for the Cenacle.